CELL-BASED DIAGNOSTIC OF BRONCHIAL CARCINOMA: CASE STUDY OF A DIAGNOSTIC VALUE OF TESTS OF CIRCULATING TUMOR CELLS AND ORGANOID CULTURE
Diagnostic based on analysis of living tumor cells is frequently used in oncology. Circulating tumor cells in the blood and cells obtained from a tumor biopsy are used to access their carcinogenic properties and subsequently to predict possible development of the disease. Here we report use of these two tests to assess aggressiveness and metastatic potential of a bronchial adenocarcinoma. The circulating tumor cells test was negative, no circulating cells was observed. It indicates that there was no metastatic spread. However, test with the surgery biopsy showed presence of aggressive cellular clones. The tumor cells from the biopsy proliferated and spread from the cultured tissue. Moreover, the tumor cells formed colonies of cells which lost contact inhibition. This is an indication of aggressive carcinogenic features of the cells in tumor organoids. Combination of both tests showed that the local tumor had an aggressive phenotype, but no detectable spreading of cells. Therefore, these tests support a management plan with removal the primary tumor and regular monitoring, without need of an extensive chemotherapy.
Millner L.M., Linder M.W., Valdes R. Jr. Circulating tumor cells: a review of present methods and the need to identify heterogeneous phenotypes. Ann Clin Lab Sci. 2013;43(3):295-304.
Maly V., Maly O., Kolostova K., Bobek V. Circulating Tumor Cells in Diagnosis and Treatment of Lung Cancer. In Vivo. 2019 Jul-Aug;33(4):1027-1037. https://doi.org/10.21873/invivo.11571
Abalde-Cela S., Piairo P., Diéguez L. The Significance of Circulating Tumour Cells in the Clinic. Acta Cytol. 2019;63(6):466-478. https://doi.org/10.1159/000495417
Pawlikowska P., Faugeroux V., Oulhen M., Aberlenc A., Tayoun T., Pailler E., Farace F.. Circulating tumor cells (CTCs) for the noninvasive monitoring and personalization of non-small cell lung cancer (NSCLC) therapies. J Thorac Dis. 2019 Jan;11(Suppl 1):S45-S56. https://doi.org/10.21037/jtd.2018.12.80
Riethdorf S., O'Flaherty L., Hille C., Pantel K. Clinical applications of the CellSearch platform in cancer patients. Adv Drug Deliv Rev. 2018 Feb 1;125:102-121. https://doi.org/10.1016/j.addr.2018.01.011
Lerescu L., Tucureanu C., Caraş I., Neagu S., Melinceanu L., Sălăgeanu A. Primary cell culture of human adenocarcinomas--practical considerations. Roum Arch Microbiol Immunol. 2008 Jul-Dec;67(3-4):55-66.
Hynds R.E., Ben Aissa A, Gowers KHC, et al. Expansion of airway basal epithelial cells from primary human non-small cell lung cancer tumors. Int J Cancer. 2018;143(1):160-166. https://doi.org/10.1002/ijc.31383
Yoshida G.J. Applications of patient-derived tumor xenograft models and tumor organoids. J Hematol Oncol. 2020;13(1):4. Published 2020 Jan 7. https://doi.org/10.1186/s13045-019-0829-z
Yang C., Xia BR, Jin WL, Lou G. Circulating tumor cells in precision oncology: clinical applications in liquid biopsy and 3D organoid model. Cancer Cell Int. 2019;19:341. Published 2019 Dec 18. https://doi.org/10.1186/s12935-019-1067-8
Nagaraj AS., Bao J., Hemmes A., Machado M., Närhi K., Verschuren EW. Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing. J Vis Exp. 2018 Nov 29;(141). https://doi.org/10.3791/58569
Fouad YA., Aanei C. Revisiting the hallmarks of cancer. Am J Cancer Res. 2017;7(5):1016-1036.
Hanahan D., Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011 Mar 4;144(5):646-74. https://doi.org/10.1016/j.cell.2011.02.013
Chowell D., Napier J., Gupta R., Anderson K.S., Maley C.C., Sayres MAW. Modeling the Subclonal Evolution of Cancer Cell Populations. Cancer Res. 2018;78(3):830-839. https://doi.org/10.1158/0008-5472.CAN-17-1229
Loeb LA, Kohrn BF, Loubet-Senear KJ, Dunn YJ, Ahn EH, O'Sullivan JN, Salk JJ, Bronner MP, Beckman RA. Extensive subclonal mutational diversity in human colorectal cancer and its significance Proceedings of the National Academy of Sciences Dec 2019, 116 (52) 26863-26872; https://doi.org/10.1073/pnas.1910301116
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