CYTOKINE PROFI LE IN PATIENTS WITH REACTIVE ARTНRITIS CAUSED BY EPSTEIN-BARR VIRAL AND CHLAMYDIAL TRACHOMATIS INFECTIONS
Introduction. Currently, reactive arthritis is the most common joint disorder among people of working age. The infl ammatory process occurs not only as a result of bacterial infection (urogenital, intestinal or respiratory), but is also caused by viruses (viruses of hepatitis, rubella, herpesviruses, especially EBV, etc.).
Methods. 97 patients with ReA have been examined (28 patients with C. Trachomatis, 33 patients with EBV, 19 patients with combined EBV and C. Trachomatis infection, 17 patients ReA with induced by tonsillitis). The level of IL-10, IL-17, IL- 23, TNF-α and IFN-γ cytokines has been evaluated in duplicates in blood serum by fl ow cytometry technique, using magnetic microspheres conjugated to monoclonal antibodies with the help of the BioPlex 200 platform with HRF (Bio-Rad, USA) using Luminex xMAP® technology and relevant special kits.
Results. We have found a signifi cant increase in the level of TNF-α, IL-17, IL-23 and INF-γ and a decrease in the level of IL-10 in all study groups of patients with combined EBV and C. Trachomatis infection, EBV, C. Trachomatis, ReA induced by tonsillitis as compared with the control group of healthy patients.
Conclusions. Assessment of the infl ammatory process activity level by determining the concentration of cytokines enables to predict the risk of rheumatoid arthritis or other systemic autoimmune pathology in the future.
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