PECULIARITIES OF MOLECULAR PROFILES OF PATIENS WITH ALLERGY – RESIDENS OF LVIV CITY AND REGION
Introduction. Methods of investigation and knowledge in the area of allergopathy diagnosis are constantly improving. According to the consensus WAO-ARIA-GALEN, molecular diagnostics is a modern method, which implies determination of body sensitization to different isolated and purified recombinant and native proteins. Knowledge of a patient’s molecular profile enables to improve the accuracy of the diagnosis and thus to determine etiological therapeutic measures and prophylactic recommendations for lifestyle and improvement of its quality.
The aim of our research was to analyze peculiarities of molecular profiles of patients with allergy – residents of Lviv city and region.
Materials and methods. An investigation group included 100 patients with allergy symptoms, 52.0% of women and 48.0% of men, aged 25.29.5 years, 39.0% of individuals were residents of Lviv and 51.0% – residents of rural areas in Lviv region. General clinical and instrumental investigations were performed. Specific allergological investigations were performed – skin prick tests to aeroallergens (Inmunotek, Spain), total serum IgE, extracts of allergens and specific IgE to allergen components, which were detected by means of colorimetric enzymatic assay (Macro Array Diagnostic) ALEX, Austria.
Results of investigations and their discussion. By the results of skin prick-tests, the highest (56.0%) sensitization level was to an extract of herb mixture, the lowest (20.0%) – to an extract of mould. Polysensitization was detected in 52.0% of patients, mono – in 6.0%, results of skin prick-test were negative in 5.0% of patients.
Results of molecular investigations: polysensitization – in 77.0% of individuals, monosensitization – in 11.0%, and in 12.0% – ALEX was negative. Peculiarities of monosensitization – it was most often (37.0%) detected to meat allergens and aeroallergens of herb pollen (27.0%); it was observed only in urban residents to fish and domestic animals. Hyper-IgE-syndrome was detected in 45.0% of individuals (from 138.2 IU/ml to 2315.7 IU/ml). Among groups of allergen sources, sensitization was the most common to herb pollen (49.0%), trees (43.0%), domestic animals (38.0%), house dust mites (37.0%), and the most common – to milk allergens (3.0%), cockroaches (2.0%) and other allergens (1.0%). Distribution of protein groups by the incidence of detection was the following: PR 10 (30.0%), NPC2 (28.0%), lipocalins (16.0%), polcalcins (15.0%) and profilins (14.0%).
Conclusions. Component investigation of sensitization profile of patients with allergy enables to suggest personal recommendations for patients’ lifestyle, administer etiotropic allergen immunotherapy with prognosis of its efficacy.
Arlian L.G., Platts-Mills T.A. The biology of dust mites and the remediation of mite allergens inallergic disease. J Allergy Clin. Immunol 2001; 107 (3): 406-413.
Asam C., Hofer H., Wolf M., Aglas L., Wallner M. Tree pollen allergens an update from a molecular perspective. Allergy. 2015;70(10): 1201-1211.
Bousquet J., Heinzerling L., Bachert C. Practical Guide To Skin Prick Tests In Allergy To Aeroallergens. Allergy. 2012; 67(1): 18-24.
Canonica G.W, Ansotequi I.J., Pawankar R., Schmid-Grendelmeier P., Marianne van Hage, Baena-Cagnani C., Melioli G., Nunes C., Passalacqua G. A WAO-ARIA-GA2LEN consensus document on molecular-based allerdy diagnostics. World Allergy Organ. J 2013; 6:13.
Chen K.W., Blatt K. Der p1/Der p2 combination vaccines for immunotherapy of house dust mite allergy. J Allergy Clin. Immunol 2012; 130 (2): 435-443.
D’Amato G., Cecchi L., Bonini S., Nunes C., Annesi-Maesano I., Behrendt H. Allergenic pollen and pollen allergy in Europe. Allergy. 2007; 62(9): 976-990.
De Vouge M.W., Thaker A.J., Zhang L., Muradia G., Rode H., Vijay H.M. Molecular cloning of IgE-binding fragments of Alternaria alternata allergens. Int Arch Allergy Immunol. 1998; 116(4): 261-8
Fiocchi A., Terracciano L., Bouygue G.R., Veglia F., Sarratud T., Martelli A., Restani P. Incremental prognostic factors associated with cow’s milkallergy outcomes in infant and child referrals: the Milan Cow’s MilkAllergy Cohort study. Ann Allergy Asthma Immunol. 2008; 101: 166-173.
Freeman T.M. Clinical practice. Hypersensitivity to Hymenoptera stings. N Engl J Med. 2004;351: 1978-84.
Fuentes A.V., Sanchez M.I., Perez M.A., Baeza M.L., de Barrio F.M.: Allergy to mammal’s meat in adult life: immunologic and follow-up study. J Investig Allergol Clin Immunol .2005; 15(3): 228-231.
Golden D.B., Kelly D., Hamilton P.G., Craig T.J. Vonom immunotherapy reduces large local reactions to insect stings. J Allergy Clin Immunol. 2009; 123: 1371-5.
Ipsen H., Bøwadt H., Janniche H., Nüchel Petersen B., Munch E.P., Wihl J.A., Løwenstein H. Immunochemical Immunochemical Characterization of Reference Alder (Alnus glutinosa) and Hazel (Corylus avellana) Pollen Extracts and the Partial Immunochemical Identity between the Major Allergens of Alder, Birch and Hazel Pollens. Allergy. 1985; 40(7): 510-518.
Madhurantakam C., Nilsson B., Uchtenhagen H., Konradsen J., Saarne T., Hogbom E., Sandalova T., Gronlund H., Achour A.: Crystal structure of the dog lipocalin allergen Can f 2: implications for cross-reactivity to the cat allergen Fel d 4. J Mol Biol. 2010; 401: 68-83.
Niederberger V., Purohit A., Oster J.P., Spitzauer S., Valenta R., Pauli G. The allergen profile of ash (Fraxinus excelsior) pollen: cross-reactivity with allergens from various plant species. Clinical and Experimental Allergy. 2002; 32: 933-941.
Palomares O., Villalba M., Quiralte J., Polo F., Rodr?guez R. 1,3-beta-glucanases as candidates in latex-pollen-vegetable food cross-reactivity. Clinical and Experimental Allergy. 2005; 35(3): 345-51.
Panzner P., Vachová M., Vítovcová P., Brodská P., Vlas T. A Сomprehensive Analysis of Middle-European Molecular Sensitization Profiles to Pollen Allergens. Int Arch Allergy Immunol. 2014; 164: 74-82.
Pichler U., Hauser M., Wolf M. Pectate Lyase Pollen Allergens: Sensitization Profiles and Cross-reactivity Pattern. PLoS One. 2015; 10: 1-19.
Reininger R., Varga E.M., Zach M., Balic N., Lindemeier A.D., Swoboda I., Grцnlund H., van Hage M., Rumpold H., Valenta R., Spitzauer S.: Detection of an allergen in dog dander that cross-reacts with the major cat allergen, Fel d 1. Clin Exp Allergy 2007; 37: 116-124.
Spitzauer S., Schweiger C., Anrather J., Ebner C., Scheiner O., Kraft D. Characterisation of dog allergens by means of immunoblotting. Int Arch Allergy Immunol 1993; 100: 60-7.
Zubchenko S.O., Yuryev S.D. Allergy to pets: differential approaches to selection of AIT prescription with due account of component studies Quest J. Journal of Medical and Dental Science Reseach. 2016; 4: 29-35.
Zubchenko S.O., Yuryev S.D. Assesment diagnostic criteria in patients with different clinical and laboratory manifestations of sensitization to hous dust mites efficiency and forecasting allergen specific immunotherapy based on allergies component diagnostic. Allergology and Clinical Immunology. 2015; XXI: 153-160.
Weber R.W. Alternaria alternata. Ann Allergy Asthma Immunol. 2001; 87(5): A-4.
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