• Andriana Belyak Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • Oksana Zayachkivska Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Keywords: biofluids, saliva, non-invasive samples, crystalisation, stress resistibility, ovulation time


Aim: The aim of the study was to reveal the relationship between stress resistibility levels and
saliva microcrystallization and to investigate whether menstruation phase has infl uence on the
saliva microcrystalisation.
Methods: The group of obseravtion included 26 healthly women aged 19-30. Facies of saliva
were investigated by microscopic analysis of hydration of drops of mixed saliva (Havriluk, 2014).
For estimation of stress resistibility levels questionnaire created by ISMA (International stress
management association, 2011) was used. To reveal the infl uence of menstrual phase on the
microcrystalisation saliva facies were collected from women every three days during the menstrual
Results: It was discovered that the absence of microcrystalisation (4th type) was most
often seen among people with low stress resistibility. What about relationship between saliva
microcrystalisation and menstrual cycle there was the trend of high incidence of 1st and 2nd
types during the ovulation.
Conclusions: Saliva investigation of microcrystallization types can be used as an additional
method of estimation of stress and as a method of the approximate detection of the ovulation, so
it can be used for planning pregnancy. Method of evaluation of saliva microcrystallization types is
a helpful tool for detection of characteristic of hormonal cycle and can be used for patients with
secondary amenorrhea, surgical menopause and prescription of hormonal replacement treatment
or specifi c treatment (e.g. chemotherapy).


Download data is not yet available.


Maercker A, Brewin CR, Bryant RA, Cloitre M, Reed GM, van Ommeren M, Humayun A, Jones LM, Kagee A, Llosa AE, Rousseau Proposals for mental disorders specifically associated with stress in the International Classification of Diseases-11. The Lancet. 2013 May 11;381(9878):1683-5.

Cozma S, Dima-Cozma LC, Ghiciuc CM, Pasquali V, Saponaro A, Patacchioli FR. Salivary cortisol and α-amylase: subclinical indicators of stress as cardiometabolic risk. Brazilian Journal of Medical and Bio- logical Research. 2017;50(2).×20165577

Petrakova L, Doering BK, Vits S, et al. Psychosocial stress increases salivary alpha-amylase activity independently from plasma noradrenaline levels. Schmidt U, ed. PLoS ONE. 2015;10(8):e0134561. doi:10.1371/journal.pone.0134561.

Vineetha R, Pai K-M, Vengal M, Gopalakrishna K, Narayanakurup Usefulness of salivary alpha amylase as a biomarker of chronic stress and stress related oral mucosal changes – a pilot study. J Clin Exp Dent 2014;6(2):e132-e137. doi:10.4317/jced.51355.

Lakhtin , Moskalenko P., Levkov A., & Smeyanov Y. Application of saliva crystalloscopy methods in the diagnosis of malignant tumours of salivary glands and Sjögren disease (Doctoral dissertation, Published by IASHE London), 2015.

Wiley JW, Higgins GA, Athey Stress and glucocorticoid receptor transcriptional programming in time and space: Implications for the brain–gut axis. Neurogastroenterol Mot. 2016;28(1):12-25.

Zayachkivska O.S. The role of salivary endogenic bioregulators in the formation esophagoprotection at experimental injury of the esophagus. Contemporary Gastroenterology 2006;30 (4):65-71. (In Ukrainian).

Bazin D, Letavernier E, Haymann Biomineralization versus microcrystalline pathologies: Beauty and the beast. Comptes Rendus Chimie. 2016;19(11):1395-403.

Mori , Ishikawara F., Tomoda T., Yamada S., Okamoto M., Itabashi H., Sumino H. Use of capillary electrophoresis with dual-opposite end injection for simultaneous analysis of small ions in saliva samples from wrestlers undergoing a weight training program. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1012:178-185.

Choi Saliva diagnostics integrate dentistry into general and preventive health care. Int J Prosthodont. 2010;23(3):189

Wong T. Salivary diagnostics powered by nanotechnologies, proteomics and genomics. J Am Dent Assoc. 2006;137(3):313

Tominaga, , Fujikawa, Y., Tsumoto, C., Kadouchi, K., Tanaka, F., Kamata, N., Arakawa, T. Disorder of autonomic nervous system and its vulnerability to external stimulation in functional dyspepsia. J Clin Biochem Nutr. 2016;58(2):161-5. doi: 10.3164/jcbn.15-140.

Bonaz B. Stress and the Gastrointestinal System. In Neuro-Immuno- Gastroenterology 2016 (pp. 123- 156). Springer International

Brzozowski B, Mazur-Bialy A, Pajdo R, Kwiecien S, Bilski J, Zwolinska-Wcislo M, Mach T, Brzozowski Mechanisms by which Stress Affects the Experimental and Clinical Inflammatory Bowel Disease (IBD): Role of Brain-Gut Axis. Curr Neuropharmacol 2016;14(8):892-900. Stevens RG, Zhu Y. Electric light, particularly at night, disrupts human circadian rhythmicity: is that a problem? Phil Trans R Soc B 2015 May 5;370(1667):20140120.

Proper K. , Singh A. S., Van Mechelen W., Chinapaw M. J. Sedentary behaviors and health outcomes among adults: a systematic review of prospective studies. Am J Prev Med. 2011;40(2): 174-182.

Thorp A., Owen N., Neuhaus M., Dunstan D. W. Sedentary behaviors and subsequent health outcomes in adults: a systematic review of longitudinal studies, 1996–2011. Am J Prev Med. 2011;41(2):207- 215.

Haruma , Kinoshita Y., Sakamoto S., Sanada K., Hiroi S., Miwa H. Lifestyle factors and efficacy of life- style interventions in gastroesophageal reflux disease patients with functional dyspepsia: primary care perspectives from the LEGEND study. Internal Medicine. 2015;54(7):695-701.

Kroll JL, Werchan CA, Reeves AG, Bruemmer KJ, Lippert AR, Ritz Sensitivity of salivary hydrogen sulfide to psychological stress and its association with exhaled nitric oxide and affect. Physiology & Be- havior. 2017 Oct 1;179:99-104.

Wiegand C, Savelsbergh A, Heusser MicroRNAs in Psychological Stress Reactions and Their Use as Stress-Associated Biomarkers, Especially in Human Saliva. Biomedicine Hub. 2017;2(3).

Havryliuk S., Kindrat A.B., Cymbalista I.V. Clinical value of crystallization of saliva in patients with acid-dependent diseases. Contemporary gastroenterology. 2014 №6(80) [In Ukrainian]

Abstract views: 60
PDF Downloads: 41 PDF Downloads: 0
How to Cite
Belyak A, Zayachkivska O. HOW TO ANALYZE TEST OF SALIVA MICROCRYSTALISATION AS A PREDICTOR OF STRESS RESISTIBILITY AND AN APPROXIMATE OVULATION TIME?. Proc Shevchenko Sci Soc Med Sci [Internet]. 2017Dec.29 [cited 2021Apr.14];50(2):105-9. Available from:

Most read articles by the same author(s)

1 2 > >>