HOW TO ANALYZE TEST OF SALIVA MICROCRYSTALISATION AS A PREDICTOR OF STRESS RESISTIBILITY AND AN APPROXIMATE OVULATION TIME?

Aim: The aim of the study was to reveal the relationship between stress resistibility levels and saliva microcrystallization and to investigate whether menstruation phase has infl uence on the saliva microcrystalisation. Methods: The group of obseravtion included 26 healthly women aged 19-30. Facies of saliva were investigated by microscopic analysis of hydration of drops of mixed saliva (Havriluk, 2014). For estimation of stress resistibility levels questionnaire created by ISMA (International stress management association, 2011) was used. To reveal the infl uence of menstrual phase on the microcrystalisation saliva facies were collected from women every three days during the menstrual cycle. Results: It was discovered that the absence of microcrystalisation (4th type) was most often seen among people with low stress resistibility. What about relationship between saliva microcrystalisation and menstrual cycle there was the trend of high incidence of 1st and 2nd types during the ovulation. Conclusions: Saliva investigation of microcrystallization types can be used as an additional method of estimation of stress and as a method of the approximate detection of the ovulation, so it can be used for planning pregnancy. Method of evaluation of saliva microcrystallization types is a helpful tool for detection of characteristic of hormonal cycle and can be used for patients with secondary amenorrhea, surgical menopause and prescription of hormonal replacement treatment or specifi c treatment (e.g. chemotherapy). Kye words: biofl uids, saliva, non-invasive samples, crystalisation, stress resistibility, ovulation time ЯК ІНТЕРПРЕТУВАТИ МІКРОКРИСТАЛІЗАЦІЮ СЛИНИ ЯК ПРЕДИКТОРА ОПІРНОСТІ ДО СТРЕСУ ТА ПРИБЛИЗНОГО


Results:
It was discovered that the absence of microcrystalisation (4th type) was most often seen among people with low stress resistibility. What about relationship between saliva microcrystalisation and menstrual cycle there was the trend of high incidence of 1st and 2nd types during the ovulation.
Conclusions: Saliva investigation of microcrystallization types can be used as an additional method of estimation of stress and as a method of the approximate detection of the ovulation, so it can be used for planning pregnancy. Method of evaluation of saliva microcrystallization types is a helpful tool for detection of characteristic of hormonal cycle and can be used for patients with secondary amenorrhea, surgical menopause and prescription of hormonal replacement treatment or specifi c treatment (e.g. chemotherapy).  [3,4,5,6]. Determining personal stress level in real time could be of special interest in health monitoring, wellbeing and early prediction of stress-associated disorders. Recent data suggests that biofl uids are very promised for health monitoring and creation non-invasive diagnostic tool for personal control and prevention decline of natural stress-resistibility. Recent data has shown that saliva secretome study is growing body of evidences for its using, as easy and early diagnostic tool [7,8,9,10,11].
Crystallization of saliva is the process of formation of solid crystals precipitating from a solution in which a solute from a liquid solution to a pure solid crystalline phase occurs. Crystallization is therefore an aspect of precipitation, obtained through a variation of the solubility conditions of the solute in the solvent, as compared to precipitation resulting from chemical reaction [12,13,14].
The crystallization process consists of two major events: nucleation and crystal growth. Nucleation is the step in which the solute molecules dispersed in the solvent (water) start to gather into clusters. However, if the clusters are not stable, they redissolve. The crystal growth is the subsequent growth of the nuclei that succeed in achieving the critical cluster size. Once the fi rst small crystal -the nucleus -forms, it acts as a convergence point for molecules of the solute touching or adjacent to the crystal so it increases its own dimension in successive layers. Nucleation and growth continue to occur simultaneously as long as supersaturation exists. Su-persaturation refers to a solution that contains more of the dissolved material than could be dissolved by the solvent and thus, the rate of nucleation and growth is driven by the existing supersaturation of the solution. The level of supersaturation can be increased by, among other methods, allowing the solution to evaporate. Depending on the conditions, either nucleation or growth may be predominant and as a result, crystals with different sizes and shapes are obtained. The nature of the crystallization process is governed by both thermodynamic and kinetic factors (motion of the molecules and the forces and energy acting on them), which can make it highly variable and diffi cult to control [15,16,17].
It is usually much easier to dissolve a perfect crystal in a solvent than to grow a good crystal again from the resulting solution, which means that the nucleation and growth of a crystal are primarily under kinetic, rather than thermodynamic control (18). Thus, we hypothesized that study of biophysical and functional properties of saliva microcrystallines regarding to the hormonal control would be helpful methods of personalized control of health and wellbeing.
Aim: The aim of the study was to reveal the relationship between stress resistibility levels and saliva microcrystallization and to investigate whether menstruation phase has infl uence on the saliva microcrystalisation.

Methods:
The group of obseravtion included healthy 26 women aged 19-30.
Facies of saliva were investigated by dehydration of drops of mixed saliva (19,20) and 4 types saliva of microcrystallization were indentifi ed microscopic analysis (Fig. 1). For estimation of stress resistibility levels questionnaire created by ISMA (International stress management association, 2011) was used.
To reveal the infl uence of menstrual phase on the microcrystalisation saliva facies were collected from women every three days during the menstrual cycle 3d, 6th, 9th, 13th, 18th days (Fig. 2). Results: It was discovered that the absence of microcrystalisation (4th type) was most often seen among people with low stress resistibility (44,4%), while there were 35,3% of 4th type among people with medium resistibility and no this type among people with high resistibility (Fig. 3). What about relationship between saliva microcrystalisation and menstrual cycle, all types of crystalisation were seen with the equal incidence with the trend of high incidence of 1st and 2nd types during the ovulation (Fig. 4).

Conclusions:
Saliva investigation of microcrystallization types can be used as an additional method of estimation of stress and as a method of the approximate detection of the ovulation, so it can be used for planning pregnancy.
Method of evaluation of saliva microcrystallization types is a helpful tool for detection of characteristic of hormonal cycle and can be used for patients with secondary amenorrhea, surgical menopause and prescription of hormonal replacement treatment or specifi c treatment ( e.g. chemotherapy). This method is very simple, cheap and noinvasive, so it can be used widely.